茶之理 · The Science

The Science of Green-Tea Fixation

How heat deactivates the leaf's enzyme in minutes, halting oxidation and locking in green tea's colour and freshness.

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The Science of Green-Tea Fixation
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L1 · Overview

Fresh tea leaves contain an enzyme called polyphenol oxidase (PPO) that oxidises tea polyphenols — the same reaction that reddens black tea. To keep green tea green, makers heat the freshly plucked leaf quickly to switch this enzyme off.

Pan-fired green tea dry leaf and liquor
Pan-fired green tea: the dry leaf and its clear, pale-green liquor (Lu’an Melon Slice) · Photo / Pixabay

This step is called kill-green (fixation). Enzymes are proteins, and heat denatures them irreversibly — much like a cooked egg cannot return to liquid. Once deactivated, oxidation stops, chlorophyll and fresh-tasting compounds are preserved, and the liquor stays clear green.

How heat deactivates the enzyme

Drag the slider to raise leaf temperature; watch enzyme activity and leaf colour ↓

Enzyme activity

Schematic, not exact values. Real fixation also depends on moisture, time and leaf thickness.

Common methods are pan-firing and steaming (typical of Japanese green tea). Both aim for the same thing: heat fast and thoroughly to stop the enzyme before the leaf oxidises.

L2 · Deep Dive

The four steps of green tea

01
Plucking
Leaf leaves the plant; enzymes active
02
Fixation
Heat deactivates enzymes, halts oxidation
03
Rolling
Ruptures cells, shapes the leaf
04
Drying
Sets shape, develops aroma, preserves

PPO and peroxidase (POD) catalyse the enzymatic oxidation of catechins. Rapid heating disrupts these enzymes’ protein structure and deactivates them, halting the oxidation cascade. Leaf temperatures above roughly 80 °C (176 °F), held long enough, are generally taken to deactivate most PPO. (Exact figures vary by study — needs verification with cited sources.)

“Green tea isn’t unchanged — it’s change with the pause button pressed.”

Typical flavour

Pan-fired green tea · flavour sketch

Brisk
Fresh
Bitter
Body

See also

The full process & parameters

StepParametersRole
Spreadingroom temp, 4–8 h, ~10–15% water losssheds some water, softens the leaf; slight enzyme activity
Kill-green ⭐pan ~200–300 °C, 1–3 minheat deactivates PPO/POD, halts oxidation, fixes the green; drives off grassy notes
Rollinglight→medium→heavy, 5–30 minruptures cells, shapes the leaf, aids extraction
Dryingbake / pan / sun, to 5–7% moistureends residual reactions, sets shape, develops aroma (Maillard)

Representative ranges; they vary widely by cultivar, origin and process (synthesised from standard tea-processing texts).

References

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